Drug Metabolism in Adult Rat Hepatocytes in Primary Monolayer Culture
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چکیده
منابع مشابه
Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbecco's medium supplemented with 17% fetal calf serum. The ultrastructure of monolayer cells was examined after various incubation periods. Within 4 h of plating, the isolated spherical cells adhere to the plastic surface, establish their first contacts by numerous i...
متن کاملThyroid thermogenesis in adult rat hepatocytes in primary monolayer culture: direct action of thyroid hormone in vitro
We have studied the effect of 3,5,3'-triiodothyronine (T3) on the respiration of adult rat hepatocytes in primary monolayer culture prepared from hypothyroid rat liver. After addition of T3 to the culture medium at a concentration of 2 x 10(-7) M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72--96 h, relative to control cultures (38.0 +/- 1.8 vs. 25....
متن کاملThyroid Thermogenesis in Adult Rat Hepatocytes in Primary Monolayer Culture Direct Action of Thyroid Hormone In Vitro
We have studied the effect of 3,5,3 't r i iodothyronine (Ts) on the respiration of adul t rat hepatocytes in pr imary monolayer culture p repared f rom hypothyroid rat liver. After addit ion of T3 to the culture medium at a concentration of 2 x 10 -7 M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72-96 h, relative to control cultures (38.0 -+ 1.8 vs...
متن کاملThyroid Thermogenesis in Adult Rat Hepatocytes in Primary Monolayer Culture Direct Action of Thyroid Hormone In Vitro
We have studied the effect of 3,5,3 't r i iodothyronine (Ts) on the respiration of adul t rat hepatocytes in pr imary monolayer culture p repared f rom hypothyroid rat liver. After addit ion of T3 to the culture medium at a concentration of 2 x 10 -7 M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72-96 h, relative to control cultures (38.0 -+ 1.8 vs...
متن کاملSpecies differences in the metabolism of 2-acetylaminofluorene by hepatocytes in primary monolayer culture.
Monolayers of hepatocytes from mouse, hamster, rat, and guinea pig metabolized 2-acetylaminofluorene (AAF) to ether-extractable, water-soluble as well as covalently macromolecular bound products. Hamster hepatocytes showed the highest rate of formation of ether-extractable metabolites, rat and guinea pig the lowest. These species differences reflected mainly differences in the formation of 2-am...
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ژورنال
عنوان ژورنال: Gastroenterology
سال: 1977
ISSN: 0016-5085
DOI: 10.1016/s0016-5085(77)80017-6